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T-Select Human CD1d Tetramer-SA-PE

50 tests



The α3 mutation significantly reduces the binding of MHC tetramer to human CD8. To minimizes the aberrant effect of some CD8 antibodies; clone SFCI21Thy2D3 (also known as “T8”) is recommended.

TargetNKT cells
Product TypeTetramer
Species ReactivityHuman
Research AreaImmunology, Immune Monitoring
SpecificityNKT cells
FormulationThe reagent is dissolved in aqueous buffer that contains 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5 mM EDTA, 0.2% BSA and 0.09% NaN3. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.
Description/BackgroundNatural Killer T (NKT) cells, a type of T cell that plays a significant role in immune response, produces a large quantity of INF- and IL-4 in response to glycolipids that presented by CD1d molecules. In recent years, NKT cells are reported to play a part in diabetes and tumor immunity. Therefore, a technology allowing quantitative measurement of CD1d-positive NKT cells would be a useful tool for immunology and clinical laboratory examinations. The development of MHC Tetramer technology has provided a breakthrough in the ability to follow T cell populations defined by their antigen specificity. Tetramers have been used widely to obtain a detailed analysis of the distribution and frequency of conventional CD4+ and CD8+ antigen-specific T cells during a variety of immune responses. T-Select Human CD1d Tetramer is a reagent created by tetramerizing biotinylated human CD1d/2m complexes with streptavidin labeled phycobiliprotein. -galactosylceramide (-GalCer), a glycosphingolipid originally isolated from marine sponges, appears to be presented by CD1d to activate both human and mouse NKT cells. -GalCer loaded T-Select Human CD1d Tetramer is a highly specific reagent for detection of NKT cells. Measurement can be performed using isolated lymphocytes/monocytes.
Storage TemperatureStore protected from light at 2-8ºC. Do not freeze.
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
50 tests


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