Human CD1d Tetramer (α-GalCer loaded)-PE
The α3 mutation significantly reduces the binding of MHC tetramer to human CD8. To minimizes the aberrant effect of some CD8 antibodies; clone SFCI21Thy2D3 (also known as “T8”) is recommended.
| Target | NKT cells |
|---|---|
| Product Type | Tetramer |
| Application | FCM |
| Conjugate | PE |
| Class | Class I |
| Allele | CD1d |
| Research Area | Immunology, Immune Monitoring |
| Protocols | FCM |
| Specificity | NKT cells |
| Formulation | The Tetramer is dissolved in an aqueous buffer containing 0.5 mM EDTA, 0.2% BSA, 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 0.09% NaN3. |
| Description/Background | Natural Killer T (NKT) cells, a type of T cell that plays a significant role in immune response, produces a large quantity of INF-gamma and IL-4 in response to glycolipids that presented by CD1d molecules. In recent years, NKT cells are reported to play a part in diabetes and tumor immunity. Therefore, a technology allowing quantitative measurement of CD1d-positive NKT cells would be a useful tool for immunology and clinical laboratory examinations. The development of MHC Tetramer technology has provided a breakthrough in the ability to follow T cell populations defined by their antigen specificity. Tetramers have been used widely to obtain a detailed analysis of the distribution and frequency of conventional CD4+ and CD8+ antigen-specific T cells during a variety of immune responses. T-Select Human CD1d Tetramer is a reagent created by tetramerizing biotinylated human CD1d/beta2m complexes with phycobiliprotein-labeled streptavidin. alpha-galactosylceramide (alpha-GalCer), a glycosphingolipid originally isolated from marine sponges, appears to be presented by CD1d to activate both human and mouse NKT cells. T-Select Human CD1d Tetramer (alpha-GalCer loaded) is a highly specific reagent for detection of NKT cells. Measurement can be performed using isolated lymphocytes/monocytes. |
| Storage Temperature | Store at 2 to 8°C. Do not freeze. Minimize exposure to light. The expiration date is indicated on the vial label. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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