Anti-LC3 mAb for My select sampler set
This LC3 monoclonal antibody of 20 µL is validated for multiple applications (FCM, IC, IH, IP, WB) and has over 25 citations.
More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.
| Target | LC3 |
|---|---|
| Product Type | Antibody |
| Application | FCM, ICC, IHC, IP |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 κ |
| Immunogen | Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.) |
| Host Species | Mouse |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | 40 µg IgG in 20 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Autophagy |
| Description/Background | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3. |
| Storage Temperature | -20°C |
| Protocols | FCM, ICC, IHC, IP |
| Clone Number | 4E12 |
| Concentration | 2 mg/mL |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
