Anti-Atg5
Monoclonal Antibody of 100 µL targeting Atg5 for WB.
| Target | Atg5 |
|---|---|
| Product Type | Antibody |
| Application | WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 ĸ |
| Immunogen | Recombinant Human full-length Atg5 (275 a.a.) |
| Host Species | Mouse |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Cancer, Immunology, Autophagy |
| Description/Background | Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Autophagy has two ubiquitin-like conjugation systems, the Atg12 and LC3-II systems. In the Atg12 conjugation system, the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome, the Atg12-Atg5-Atg16L complex dissociates from the membrane. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | WB |
| Clone Number | 4D3 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone 4D3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with recombinant full-length human Atg5 (275 aa). |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
