Anti-LC3 (Human) mAb (Monoclonal Antibody)
This LC3 antibody is validated for multiple applications (ICC, IH, IP, FCM, Immuno-EM) and has numerous citations. This is a monoclonal antibody targeting LC3 for FCM, ICC, IP.
More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.
| Target | LC3 |
|---|---|
| Product Type | Antibody |
| Application | FCM, ICC, IHC, Immuno-EM, IP |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 ĸ |
| Immunogen | Recombinant human LC3 (MAP1LC3B: 1-120 a.a.) |
| Host Species | Mouse |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | 200 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Autophagy |
| Description/Background | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3. |
| Storage Temperature | -20°C |
| Protocols | FCM, ICC, IHC, Immuno-EM, IP |
| Clone Number | 4E12 |
| Concentration | 2 mg/mL |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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