Anti-LC3 pAb for My select sampler set
Polyclonal Antibody of 20 µl targeting LC3 for WB, IC and IH.
| Target | LC3 |
|---|---|
| Product Type | Antibody |
| Application | IC, IH, WB |
| Clonality | Polyclonal |
| Conjugate | Unlabeled |
| Isotype | IgG |
| Immunogen | Recombinant full-length Rat LC3 |
| Host Species | Rabbit |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | 20 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Autophagy |
| Description/Background | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3. |
| Storage Temperature | This antibody is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | IC, IH, WB |
| Clone Number | Polyclonal |
| Source | This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant full-length rat LC3. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
