Anti-p62 (SQSTM1) (Human) mAb
This monoclonal Antibody targets p62 for FCM, ICC, IHC, IP, WB.
| Target | p62 |
|---|---|
| Product Type | Antibody |
| Application | FCM, ICC, IHC, IP, WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 ĸ |
| Immunogen | Recombinant human p62 (120-440 a.a.) |
| Host Species | Mouse |
| Species Reactivity | Human, Hamster, Mouse, Rat |
| Formulation | 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Autophagy |
| Description/Background | p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | FCM, ICC, IHC, IP, WB |
| Clone Number | 5F2 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone 5F2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human p62 protein corresponding to amino acids 120-440. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
