Anti-CD11b (Human/Mouse) mAb-PE-Cy7 (Monoclonal Antibody)
The M1/70 monoclonal antibody specifically reacts with the 170 kDa αM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.
M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.
Application Notes:
The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ug per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
| Target | CD11b |
|---|---|
| Product Type | Antibody |
| Application | FCM |
| Clonality | Monoclonal |
| Conjugate | PE |
| Isotype | IgG2b ĸ |
| Host Species | Rat |
| Species Reactivity | Human, Mouse |
| Formulation | Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
| Research Area | Immunology, Angiogenesis, Flow Cytometry, Innate Immunity |
| Storage Temperature | The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product. |
| Protocols | FCM |
| Clone Number | M1/70 |
| Concentration | 0.2 mg/mL |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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