Anti-His-tag mAb-Alexa Fluor® 594 (Monoclonal Antibody)
Monoclonal antibody conjugated to Alexa Fluor® 594 of 50 µl targeting His-tag for ICC, IH.
| Product Type | Antibody |
|---|---|
| Application | ICC, IH |
| Clonality | Monoclonal |
| Conjugate | Alexa Fluor® 594 |
| Isotype | IgG2a ĸ |
| Immunogen | 6xHis-tagged protein |
| Host Species | Mouse |
| Formulation | 50 µg of IgG in 50 µL volume of PBS containing 1% BSA and 0.1% ProClin150. |
| Research Area | Epitope Tag, Purification/Separation, Smart-IP |
| Description/Background | The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins. This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at 4°C. |
| Protocols | ICC, IH |
| Clone Number | OGHis |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone OGHis) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP-1 with Balb/c mouse splenocyte immunized with 6xHis tagged protein. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
