His tagged Protein PURIFICATION KIT
MBLI’s His tagged Protein PURIFICATION GEL is designed for the isolation of His tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. It can be used for 20 tests.
| Target | His |
|---|---|
| Product Type | Kit |
| Application | IP |
| Research Area | Epitope Tag, Purification/Separation, Smart-IP |
| Description/Background | <p>The hexa Histidine-tag (6xHis tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His tagged proteins. Unfortunately, some serum components are absorbed by the metal chelate affinity columns, making these columns impractical for the purification of proteins secreted into the culture<br /> supernatant. Indeed, many proteins have intrinsic histidine residues or other untagged host proteins bind the metal chelate affinity columns and elute with His tagged protein. These untagged contaminants may be removed using an additional purification or laborious optimizing the imidazole concentration.<br /> MBL’s His tagged Protein PURIFICATION KIT is designed for the isolation of His tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBL has developed the Anti-His tag Beads to purify His tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified<br /> proteins can maintain the activity and conformation. The elution of His tagged proteins from the Beads is achieved by the addition of the 6xHis peptide. As the 6xHis peptide competes with His tagged proteins on the Beads, the purified proteins do not lose the protein activity. The simple procedures of this kit have been optimized by using a Spin Column resulting in high efficiency.</p> |
| Protocols | IP |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
