HA-tagged Protein Magnetic Purification Kit

DATA SHEET

Product Description:

The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require highly purified, active proteins for studies involving signaling pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and much more.

The method of purification is one of the important keys for maintaining protein structure and function. The HA-tagged Protein Magnetic PURIFICATION KIT is designed for the isolation of HA-tagged protein from cell lysates and culture supernatant. Severe conditions such as acidic or alkaline elution can denature protein structure while maintaining a neutral pH can preserve protein activity and conformation. MBLI has developed the Anti-HA-tag Magnetic Beads to quickly and efficiently purify HA-tagged proteins at neutral pH to maintain protein activity and native conformation. The HA-tag peptide competitively elutes the HA tagged protein from Anti-HA-tag Magnetic Beads while minimally interfering with protein function.

The affinity of the anti-HA-tag antibody is also very important. Very high affinity antibody cannot be dissociated from the HA-tagged protein, and low affinity antibody is not sufficient for binding. MBLI has optimized the affinity of the anti-HA-tag antibody to permit high yields and efficient purification of functional HA-tagged proteins for biochemical studies. This kit is simple and fast, because all procedures of this kit have been optimized.