Anti-CD40 (Human) mAb Functional Grade (Monoclonal Antibody)
The 5C3 monoclonal antibody specifically reacts with human CD40, a 48kDA type I glycoprotein. CD40 is part of the TNFR family and expressed on a subset of T cells, B cells, dendritic cells, and macrophages. It has been shown to be involved in the protection of B cells from apoptosis, B cell differentiation, co-stimulation, and isotype class-switching. The 5C3 antibody partially blocks the binding of CD40L (CD154). In the presence of IL-4 or PMA, the 5C3 antibody promotes B cell proliferation.
Application Notes:
The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. It is recommended that the reagent be titrated for optimal performance for each application.
| Target | CD40 |
|---|---|
| Product Type | Antibody |
| Application | FCM, FA |
| Clonality | Monoclonal |
| Isotype | IgG1 ĸ |
| Host Species | Mouse |
| Species Reactivity | Human |
| Formulation | Phosphate-buffered aqueous solution, ph7.2. |
| Research Area | Immunology, Adaptive Immunity, Flow Cytometry |
| Storage Temperature | The product should be stored undiluted at 4°C. Do not freeze. |
| Protocols | FCM, FA |
| Clone Number | 5C3 |
| Purification | The monoclonal antibody was purified utilizing affinity chromatography |
| Endotoxin levels | The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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