Anti-DDDDK-tag pAb (Polyclonal Antibody)
Polyclonal Antibody of 100 µg targeting DDDDK-tag for ICC, IP, WB.
| Target | DDDDK |
|---|---|
| Product Type | Antibody |
| Application | ICC, IP, WB |
| Clonality | Polyclonal |
| Conjugate | Unlabeled |
| Isotype | IgG |
| Immunogen | Synthetic peptide binding KLH, DYKDDDDK |
| Host Species | Rabbit |
| Formulation | PBS/50% glycerol, pH 7.2 |
| Research Area | Epitope Tag, Purification/Separation, Smart-IP |
| Description/Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. The DDDDK epitope tag peptide sequence (DYKDDDDK) was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. The DDDDK peptide has been widely used as a multi-purpose tag, and anti-DDDDK antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK fusion proteins. |
| Storage Temperature | -20°C |
| Protocols | ICC, IP, WB |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
