Anti-GST-tag mAb
Monoclonal Antibody of 100 μg targeting GST for WB.
| Target | GST |
|---|---|
| Product Type | Antibody |
| Application | WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG2b ĸ |
| Immunogen | Recombinant GST |
| Host Species | Mouse |
| Formulation | 100 g IgG in 100 L volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
| Research Area | Epitope Tag, Purification/Separation, Smart-IP |
| Description/Background | Expression vectors containing a protein and a tag protein are commonly used. Bacterial glutathione S-transferase (GST)-tag fusion protein expression system is preferably used in various laboratories because of its simple protein purification step by an affinity chromatography. This specific antibody for GST-tag fusion protein is useful tool for monitoring of the fusion protein expression and affinity purification |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | WB |
| Clone Number | 3B2 |
| Concentration | 1 mg/ml |
| Source | This antibody was purified from hybridoma (clone 3B2) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with recombinant bacterial glutathione S-transferase. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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