T-Select I-Ad OVA323-339 Tetramer-APC
MHC Tetramers are used for the detection of antigen-specific T-cell populations. MHC-peptide tetramer complexes recognize antigen specific T-cells at the single cell level, allowing for accurate measurements of the functional immune response in cancer, infectious diseases, and autoimmune diseases. Due to their increased avidity, MHC tetramers stably bind to cognate TcRs and are evaluated using flow cytometry.
| Target | OVA |
|---|---|
| Product Type | Tetramer |
| Application | FCM |
| Conjugate | APC |
| Class | Class II |
| Species Reactivity | Mouse |
| Allele | I-Ad |
| Peptide Sequence | ISQAVHAAHAEINEAGR |
| Research Area | Immunology, Immune Monitoring |
| Protocols | FCM |
| Specificity | OVA |
| Formulation | The Tetramer is dissolved in an aqueous buffer containing 0.5 mM EDTA, 0.2% BSA, 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 0.09% NaN3. |
| Description/Background | This Tetramer reagent comprises murine class II MHC I-Ad and epitope peptide derived from ovalbumin (OVA), and it can detect an I-Ad -restricted OVA323-339 -specific CD4+ T cells. The CD4+T cell epitope,OVA323-339, of the OVA model antigen has been a useful tool in immunology. The DO11.10 transgenic mouse strain carries a TCR transgene specific for the OVA323-339 peptide, and it is useful for studying T cell immunology. A Tetramer, which is constructed with the same allele (I-A d ) of interest and an irrelevant peptide, may be used as a negative control Tetramer. Alternatively, a cell population devoid of Tetramer-positive cells may be used as a negative control. |
| Storage Temperature | Store at 2 to 8°C. Do not freeze. Minimize exposure to light. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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