QuickSwitch™ Quant H-2 Kb Tetramer Kit-BV421
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 143.
Please use this link for access to the QuickSwitch™ Peptide Exchange Calculator to check your binding affinity.
| Target | Custom |
|---|---|
| Product Type | Tetramer |
| Application | FCM |
| Conjugate | BV421 |
| Class | Class I |
| Species Reactivity | Mouse |
| Allele | H-2Kb |
| Peptide Sequence | Custom |
| Research Area | Immunology, Immune Monitoring |
| Protocols | FCM |
| Specificity | Custom |
| Description/Background | <p><a href="https://www.mblintl.com/contact-us/" target="_blank" rel="noopener">Contact us</a> with any specific questions you may have!</p> |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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