| Target | CD20 |
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| Application | FC |
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| Clonality | Monoclonal |
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| Conjugate | Allophycocyanin (APC) |
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| Isotype | Human IgG1κ |
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| Immunogen | Human lymphoblastoid cell line SB. |
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| Species Reactivity | Human, Cynomolgus Monkey, Rhesus Monkey |
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| Formulation | This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. |
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| Research Area | Cancer, Immunology |
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| Description/Background | CD20 is a 33-37 kD transmembrane-spanning phosphoprotein found on the surface of developing B-cells and various B-cell malignancies. CD20 is a popular target for mAb therapy because depleting developing B-cells generally does not cause permanent side effects (due to the fact that mature plasma cells and B-cell progenitors do not express CD20 and that there is limited expression of CD20 among other cell lineages). Rituximab is a chimeric monoclonal antibody that binds to CD20. The precise function of CD20 is still unknown. However, it is suspected to play a role in Ca2+ influx across plasma membranes, maintaining intracellular Ca2+ concentration, and allowing the activation of B cells. Rituximab is used to treat some autoimmune diseases and types of cancer such as non-Hodgkin lymphoma, chronic lymphocytic leukemia, and rheumatoid arthritis among others. The Fc portion of Rituximab mediates antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Rituximab increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen) and also induces apoptosis of CD20+ cells. This ultimately results in the elimination of B cells (including the cancerous ones) from the body, and thus allows a new population of healthy B cells to develop from lymphoid stem cells. Anti-Human CD20 (Rituximab) utilizes the same variable regions from the therapeutic antibody Rituximab making it ideal for research projects. |
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| Storage Temperature | This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze. |
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| Protocols | FC |
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| Clone Number | 10F381 |
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| Concentration | 0.2 mg/ml |
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| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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