Anti-β-galactosidase (Human) pAb (Polyclonal Antibody)
Polyclonal Antibody of 100 µl targeting β-galactosidase for ICC, IHC, IP, WB.
Target | β-Galactosidase |
---|---|
Product Type | Antibody |
Application | ICC, IHC, IP, WB |
Clonality | Polyclonal |
Conjugate | Unlabeled |
Isotype | IgG |
Immunogen | Updated Immunogen to: E. coli full-length β-galactosidase |
Host Species | Rabbit |
Formulation | 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
Research Area | Cell Biology, Epitope Tag |
Description/Background | β-galactosidase is a homo-tetrameric enzyme, with each subunit having a molecular weight of 116 kDa. Eukaryotic genes are often expressed as fusion protein by the β-galactosidase (lacZ) gene, resulting in the expression of a fusion hybrid with b-galactosidase. Anti-β-galactosidase antibody provides a simple method to isolate fusion proteins directly from crude bacterial lysates, using immunoaffinity chromatography or immunoprecipitation. Anti-β-galactosidase can also be used for the immunocytochemical detection of β-galactosidase in cells and tissues that express transfected bacterial lacZ gene or β-galactosidase fusion protein. |
Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
Protocols | ICC, IHC, IP, WB |
Source | This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with full length E. coli β-galactosidase. |
Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |