| Target | Kaede |
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| Product Type | Antibody |
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| Application | WB |
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| Clonality | Polyclonal |
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| Conjugate | Unlabeled |
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| Isotype | IgG |
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| Immunogen | Full-length Kaede |
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| Host Species | Rabbit |
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| Formulation | 100 µL volume of 10 mM phosphate buffer containing 0.3 M NaCl and 0.09 % NaN3, pH 8.0. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain. |
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| Research Area | Fluorescent Proteins |
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| Description/Background | CoralHue® Kaede protein emits green fluorescence that can be converted to red. The red fluorescence is comparable in intensity to the green and is stable under usual aerobic conditions. The green-to-red conversion is highly sensitive to irradiation with UV or violet light (350-410 nm). Maximal illumination results in a 2,000-fold increase in the ratio of red-to-green signal. The excitation lights used to elicit red and green fluorescence do not induce the photoconversion. This property provides a simple and powerful technique for regional optical marking. |
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| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
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| Protocols | WB |
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| Source | This antibody was fractioned by salting out and gel filtration on a Sephadex G-200 column. The rabbit was immunized with the recombinant CoralHue® Kaede. |
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| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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