sTREM-1, Human, ELISA kit

DATA SHEET

The human sTREM-1 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.  Samples and standards are incubated in microtiter wells coated with antibodies recognizing human sTREM-1. Biotinylated tracer will bind to the captured human sTREM-1. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human sTREM-1 standards (log). The human sTREM-1 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.