Anti-Sap155 mAb (Monoclonal Antibody)
SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N-terminus and is phosphorylated prior to catalytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
This monoclonal antibody reacts with Sap155 (155 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.
| Target | Sap155 |
|---|---|
| Product Type | Antibody |
| Application | ICC, IP, WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG2b |
| Immunogen | Recombinant Sap155 (98-198 a.a.) |
| Host Species | Mouse |
| Species Reactivity | Human, Mouse |
| Formulation | 100 μg IgG in 100 μl PBS containing 50% glycerol, pH 7.2. Contains nopreservatives. |
| Research Area | Cancer, Epigenetics |
| Storage Temperature | -20°C |
| Protocols | ICC, IP, WB |
| Clone Number | 16 |
| Concentration | 1 mg/mL |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
