Anti-CD52 (CAMPATH-1) (Mouse) mAb-FITC (Monoclonal Antibody)
Monoclonal antibody conjugated to FITC of 100 µl targeting CAMPATH-1/CD52 for FCM.
| Target | CAMPATH-1/CD52 |
|---|---|
| Product Type | Antibody |
| Application | FCM |
| Clonality | Monoclonal |
| Conjugate | FITC |
| Isotype | IgG2a |
| Immunogen | Transgenic mouse spleen cell IL-2Ralpha |
| Host Species | Rat |
| Species Reactivity | Mouse |
| Formulation | 50 μg IgG in 100 μl volume of PBS containing 1% BSA and 0.09% NaN3.*Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain. |
| Research Area | Immunology, Cell Biology |
| Description/Background | CAMPATH-1, also known as CD52, is a heavily glycosylated, GPI-anchored protein expressed at high levels on almost all thymocytes, lymphocytes, monocytes, and macrophages. The function of CD52 is unknown. However, CD52 is an exceptionally good target for complement-mediated cell lysis and antibody-mediated cellular cytotoxicity. Humanized CAMPATH-1antibodies have been used therapeutically to effectively deplete lymphocytes in allogeneic bone marrow transplants, hematologic malignancies, and autoimmune diseases. Clinical trials suggest CAMPATH-1 antibodies are especially promising in the treatment of leukemia, non-Hodgkin lymphomas, and rheumatoid arthritis. The apparent size of CAMPATH-1 by SDS-PAGE is 25-29 kDa; however, the actual molecule is much smaller, ~ 8-9 kDa as confirmed by total structure analysis and mass spectrometry. |
| Storage Temperature | 4°C |
| Protocols | FCM |
| Clone Number | BTG-2G |
| Concentration | 500 μg/ml |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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