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Anti-Flavocytochrome b558 (Human) mAb (Monoclonal Antibody)

D162-3
Antibody
100 µl

$313.18

DATA SHEET

Monoclonal antibody of 100 µL targeting Flavocytochrome b for FCM.

TargetFlavocytochrome b
Product TypeAntibody
ApplicationFCM
ClonalityMonoclonal
ConjugateUnlabeled
IsotypeIgG1
ImmunogenHuman cytochrome b rich fraction
Host SpeciesMouse
Species ReactivityHuman
Formulation100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Research AreaCell Biology
Description/BackgroundThe NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Storage TemperatureThis antibody solution is stable for one year from the date of purchase when stored at -20°C.
ProtocolsFCM
Clone Number7D5
Concentration1 mg/mL
SourceThis antibody was purified from hybridoma (clone 7D5) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with the human cytochrome b rich fraction.
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
D162-3
Antibody
100 µl

$313.18

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