Anti-Proton Pump (H,K-ATPase α subunit) mAb
Monoclonal antibody targeting Proton pump (H,K-ATPase α subunit) for IHC.
| Target | Proton pump |
|---|---|
| Product Type | Antibody |
| Application | IHC, WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 |
| Immunogen | This product is an antibody obtained from a hybridoma by fusing mouse myeloma cells and spleen cells BALB / c nu / + mouse neonatal thymectomy anti-gastric wall cells to produce antibodies. |
| Host Species | Mouse |
| Species Reactivity | Human, Bovine, Mouse, Rat |
| Formulation | 6 mL of prediluted antibody from the supernatant containing 0.09% NaN3. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain. |
| Research Area | Apoptosis |
| Description/Background | Hydrogen-potassium adenosine triphosphatase (H+, K+-ATPase) belongs to a family of P-type cation-transporting ATPases that also includes Ca2+-ATPase and Na+, K+-ATPase. H+, K+-ATPase is responsible for acid secretion into the stomach and catalyzes electroneutral exchange of cytoplasmic hydrogen ions and external potassium ions coupled with ATP hydrolysis. The enzyme is found in gastric parietal cells where it is located in cytoplasmic vesicles or apical plasma membranes of the secretory canaliculus. H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic a subunit and a smaller but heavily glycosylated b subunit. The a subunit of H+, K+-ATPase has 10 transmembrane domains and contains sites for ATP binding and its acylphosphorylation, binding sites of proton pump inhibitors, and sites responsible for ion recognition. |
| Storage Temperature | This antibody solution is stable for 3 years from the date of purchase when stored at 4°C. |
| Protocols | IHC, WB |
| Clone Number | 1H9 |
| Source | This product is hybridoma (clone 1H9) supernatant. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c nu/+ mouse splenocyte with neonatal thymectomy-induced autoimmune gastritis. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
