Anti-Phospho-Syntide-2 mAb
Monoclonal Antibody of 100 μg targeting Syntide-2 for ELISA.
| Target | Syntide-2 |
|---|---|
| Product Type | Antibody |
| Application | ELISA |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG2b |
| Immunogen | a synthetic phosphopeptide Syntide-2 (PLARTL(pS)VAGLPGKK; synthetic substrate for CaM-Kinase II) |
| Host Species | Mouse |
| Formulation | Supplied in 20 mM phosphate buffer (pH 7.5), 300 mM NaCl, 50 % glycerol. |
| Research Area | Cancer, Cell Biology, Drug Discovery |
| Description/Background | Syntide-2, a peptide based on phosphorylation site two of glycogen synthase, is an exogenous substrate designed for CaM-Kinase (calcium/calmodulin dependent kinases) in the serine-threonine kinase family. CaM-kinase II is a multifunctional calcium/calmodulin dependent protein kinase involved in neuronal functions. This anti-Phospho-Syntide-2 monoclonal antibody has been validated with CaM-kinase II, however it has the potential for use in evaluating other serine threonine kinases such as CaM-Kinase IIa, PKCµ, Akt1, Akt2, Akt3, and PKA. The relative Vmax/Km ratios of the known Ca2+-dependent protein kinases for syntide-2 were determined to be as follows: protein kinase II, 100; protein kinase C, 22; phosphorylase kinase, 2; myosin light chain kinase, 0.005. |
| Storage Temperature | -20°C |
| Protocols | ELISA |
| Clone Number | MS-6E6 |
| Sensitivity | Anti-Phospho-Syntide-2 mAb (MS-6E6) detects phosphorylated Syntide-2 only when phosphorylated at serine residue, by ELISA. |
| Concentration | 1 mg/mL x 100 µL |
| Source | Monoclonal antibody is produced by immunizing mice with a synthetic phosphopeptide Syntide-2, PLARTL(pS)VAGLPGKK, which is synthetic substrate for CaM-Kinase II (Calmodulin dependent protein kinase II). IgG is purified by protein A-sepharose chromatography. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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