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Anti-Phospho-MBS (MYPT1) (Thr696) mAb

100 μg



Monoclonal Antibody of 100 μg targeting MBS/MYPT1 for ELISA, WB.

Product TypeAntibody
ApplicationELISA, WB
Immunogena synthetic peptide corresponding to residues surrounding Thr696 of Human MBS/MYPT1 (KLH-conjugated )
Host SpeciesMouse
Species ReactivityHuman, Chicken, Mouse, Rat
Formulation1.0 mg/mL in 10 mM HEPES KOH/150 mM NaCl/50 % glycerol, pH 7.5
Research AreaCell Biology

The expression of a constitutively active form of Rho-kinase induced stress fiber and focal adhesion formation in fibroblasts and an increase in the level of myosin light chain (MLC20) phosphorylation (1). These results in smooth muscle and in non-muscle cells are attributed to an increase in MLC20 phosphorylation and this is thought to reflect the inhibition of myosin phosphatase (MP). MP is composed of three subunits: a catalytic subunit of type 1 phosphatase delta isoform, PP1c delta, and two non-catalytic subunits, 110 and 20 kDa. The 110 kDa subunit is a targeting molecule and thus has been termed myosin phosphatase target subunit 1 (MYPT1) or myosin-binding subunit (MBS). MBS (MYPT1) is the key molecule involved in regulation of MP activity (2). Phosphorylation by Rho-kinase inhibited MP activity and this reflected a decrease in Vmax. Activity of MP with different substrates also was inhibited by phosphorylation. Two major sites of phosphorylation on chicken MBS (MYPT1) were Thr695 and Thr850. Various point mutations were designed for these phosphorylation sites. Following thiophosphorylation by Rho-kinase and assays of phosphatase activity it was determined that Thr695 was responsible for inhibition.

Rho-kinase, which is activated by GTP-RhoA, phosphorylated MBS (MYPT1) and consequently inactivated myosin phosphatase (MP). Over-expression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS (MYPT1) and MLC20. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

Storage Temperature-20°C
ProtocolsELISA, WB
Clone NumberAF20
Molecular Weight135 kDa
Concentration1 mg/mL x 100 µL
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
100 μg


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