CircuLex S100A12/EN-RAGE ELISA Kit Ver.2
This Kit is used for the quantitative measurement of human S100A12/EN-RAGE in serum, plasma, and other biological media.
| Target | S100A12 |
|---|---|
| Product Type | ELISA Kit |
| Application | ELISA |
| Species Reactivity | Human |
| Research Area | Immunology, Metabolism |
| Description/Background | Members of the S100 protein family are low molecular mass acidic proteins characterized by cell-type-specific expression and the presence of 2 EF-hand calcium-binding domains. The calgranulins are S100 proteins that are expressed in neutrophils, and are abundant in infiltrating monocytes and granulocytes under conditions of chronic inflammation. Hofmann et al. (1999) reported that RAGE is a central cell surface receptor for S100A12, which they referred to as EN-RAGE (Extracellular Newly identified RAGE-binding protein), and related members of the S100/calgranulin superfamily. Interaction of EN-RAGE (S100A12) with cellular RAGE on endothelium, mononuclear phagocytes, and lymphocytes triggered cellular activation, with generation of key proinflammatory mediators. In murine models, blockade of ENRAGE/RAGE quenched delayed-type hypersensitivity and inflammatory colitis by arresting activation of central signaling pathways and expression of inflammatory gene mediators (1). S100A12 was also isolated as proteins binding to three different anti-allergic drugs, amlexanox, cromolyn and tranilast, by drug-affinity chromatography (2). This finding implies that these three compounds might interact with these proteins when working as anti-allergic drugs. S100A12 serum concentrations indicate neutrophil activation in JRA (3), cystic fibrosis (4), Kawasaki disease (5) and Crohn's disease (6). Its function as a proinflammatory factor secreted by activated neutrophils makes this protein a potential target for future therapies. |
| Storage Temperature | Upon receipt store all components at 4°C. Don’t expose reagents to excessive light. |
| Protocols | ELISA |
| Sensitivity | The limit of detection (defined as such a concentration of S100A12/EN-RAGE giving absorbance higher than mean absorbance of blank plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 8.2 pg/mL of sample. |
| Measurement Range | 20 - 1,280 pg/mL |
| Specificity | The antibodies in the Circulex S100A12/EN-RAGE ELISA Kit Ver.2 only react with human S100A12 and without detectable cross-reactivities to other human S100 proteins. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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