CycLex® Chitotriosidase Fluorometric Assay Kit
The CycLex Research Product CycLex Chitotriosidase Fluorometric Assay Kit is used for the quantitative measurement of chitotriosidase activity in serum/plasma, culture supernatant of activated macrophages and other biological samples. It can be used for 100 assays.
| Target | chitotriosidase |
|---|---|
| Product Type | Kit |
| Application | ELISA |
| Research Area | Cell Biology |
| Description/Background | Elevated levels of serum chitotriosidase were also found in disorders caused by the abnormal activation of immune system, including sarcoidosis (9) and atherosclerosis (10, 11). It has been shown that chitotriosidase activity was elevated up to 55-fold in extracts of atherosclerotic tissue, showing a clear connection between chitotriosidase expression and lipid-laden macrophages inside human atherosclerotic vessel wall (10). Human chitotriosidase also associates with pathogen-driven diseases, and in particular with fungal infections, suggesting the role of this enzyme in host defense against chitin-containing pathogens (12, 13). Other clinical data for instance show that chitotriosidase activity is raised in plasma of African children infected with acute Plasmodium falciparum malaria (14). Additional evidence for a role of chitotriosidase during immunological responses is the observation that the enzyme is shortly and acutely up-regulated both at the level of mRNA and activity following stimulation with prolactin, IFN-γ, TNF α and LPS, but not with IL-10 (15, 16). In the blood stream, tissue macrophages largely secrete newly synthesized 50-kDa chitotriosidase, but about one-third is directly routed to lysosomes and proteolytically processed to the 39-kDa unit that remains catalytically active (17). A common chitotriosidase gene polymorphism leads to a null allele and therefore a defective enzyme activity. In white populations, 30% to 40% of individuals are carriers of this abnormal chitotriosidase allele and approximately 6% are homozygous (1, 18). |
| Storage Temperature | -80°C |
| Protocols | ELISA |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
