COOMASSIE Protein Staining Solution

DATA SHEET

The COOMASSIE protein staining solution is a convenient protein staining solution for SDS-PAGE gels. Its advanced formula provides rapid protein detection, enhanced sensitivity, and eliminates the need for destaining. Additionally, the washing step can be skipped. With no harmful substances like methanol and acetic acid, COOMASSIE sating solution is recognized for its safety and eco-friendly nature. Moreover, COOMASSIE is compatible with mass spectrophotometry.

Reaction Set up

1. Carefully shift COOMASSIE up and down few times before use.
   2. After SDS-PAGE has finished, remove the gel from the cassette and submerge it in a proper amount of COOMASSIE dye, enough to cover the gel. Lightly agitate the staining box for 10 to 30 minutes at  room temperature.
   3. As there is no need for washing or destaining, please continue to carefully remove the dye solution and rinse the excess dye in the gel with water.
   4. Image the gel.

Material needed but not provided :Container: box for gel staining , Shaker: orbital or rocking shaker

Important notes

1. There is no risk of excessive staining. Instead, superior visibility per band is achieved when the gels are submerged overnight.

2. Carefully shift COOMASSIE up and down few times before use.

3. If the gel is thicker than mini-gel (1mm), it will take longer to incubate.

4. The dye solution may contain blue clumps. This is normal and easily dissolved in distilled water