5X COOMASSIE Concentrate Protein Staining Solution
The 5X Concentrate COOMASSIE is a convenient and concentrated protein staining solution for SDS-PAGE gels. Its advanced formula provides rapid protein detection, enhanced sensitivity, and eliminates the need for destaining. Additionally, the washing step can be skipped. With no harmful substances like methanol and acetic acid, COOMASSIE sating solution is recognized for its safety and eco-friendly nature. Moreover, COOMASSIE is compatible with mass spectrophotometry.
Reaction Set up
Prepare a 5L bottle. Unpack and pour the liquid content into the bottle. Rinse residual reagent in the original bottle with deionized water.Add deionized water until the volume reaches 5L. Make 5X Concentrated COOMASSIE into 1X COOMASSIE protein staining solution. 2. Carefully mix COOMASSIE up and down few times before use. 3. After SDS-PAGE has finished, remove the gel from the cassette and submerge it in the proper amount of COOMASSIE stain, enough to cover the gel. Lightly agitate the staining box for 10 to 30 minutes at room temperature.
material needed but not provided :Container: box for gel staining , Shaker: orbital or rocking shaker
Important notes
1. There is no risk of excessive staining. Instead, superior visibility per band is achieved when the gels are submerged overnight. 2. Carefully shift COOMASSIE up and down few times before use. 3. If the gel is thicker than mini-gel (1mm), it will take longer to incubate. 4. The dye solution may contain blue clumps. This is normal and easily dissolved in distilled water
| Product Type | Reagent |
|---|---|
| Research Area | Western Blot Reagents |
| Storage Temperature | Stable for up to 24 months at 25°C |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
