Unstained Protein Ladder, Broad Range (12 unstained bands, 10-200 kDa)
The unstained Protein Ladder is a high-quality combination of 12 unstained recombinant proteins that cover a broad range of molecular weights from 10 to 200 kDa. The proteins can be easily distinguished as separate bands when separated on the SDS-PAGE gel (MES buffer), and the intensified 25kDa and 85kDa protein bands act as the two reference bands.
The protein ladder is an essential tool for accurately measuring protein size and ensures precise separation during SDS-polyacrylamide gel electrophoresis. The ladder provides unmatched accuracy for protein size and separation during Western transfers on PVDF, nylon, or nitrocellulose membranes.
material needed but not provided
Vertical Electrophoresis system ,Power supplies , Vortex or equivalent ,Microcentrifuge
Reaction Set up
3μl or 5μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5μl per well for general Western transferring.
| Product Type | Reagent |
|---|---|
| Formulation | 0.2-0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol |
| Research Area | Western Blot Reagents |
| Storage Temperature | Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. Stable for up to 24 months at -20°C. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
