Recombinant Mouse IL-18 (without BSA)
IL-18 Protein of 25 µg.
cDNA encoding the matured mouse IL-18 protein sequence (corresponding to 36-192 aa) was expressed in E. coli.
| Target | IL-18 |
|---|---|
| Product Type | Recombinant Protein |
| Formulation | Lyophilized in PBS containing 1% sucrose. Reconstitute in 250 µL of ice-cold distilled water on ice. |
| Research Area | Immunology, Cytokine & Growth Factors |
| Description/Background | Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon- (IFN-) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN- is produced by activated T or NK cells and plays critical roles in the defense against microbial pathogens. IFN- activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN- also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN- and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN- in T cells, enhancement of Fas ligand expression by Th1 cells. |
| Storage Temperature | Lyophilized IL-18 is stable for 24 months from the date of manufacture when store at -20°C or below. After reconstitution, avoid repeated freezing and thawing. The IL-18 can be stored for 1 week at 4°C. For storage, prepare appropriate aliquots and freeze them at -80°C using low retention tube. |
| Molecular Weight | 18 kDa |
| Purity | Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
