pFucci-G1 Orange (Expression vector)

FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enables the visualization of cell cycle progression in living cells. FUCCI utilizes the phase-dependent nature of replication licensing factors Cdt1 and Geminin. A fusion protein of a fragment of Cdt1 (amino acids 30-120) with the fluorescent protein monomeric Kusabira-Orange 2 (mKO2) serves as an indicator of G1 phase. A fusion protein of a fragment of Geminin (amino acids 1-110 or 1-60) with the fluorescent protein monomeric Azami-Green 1 (mAG1) visualizes the S,G2 and M phase. In summary, FUCCI utilizes the highly selective, rapid degradation of the replication licensing factors mediated by the ubiquitin proteasome system to give excellent visualizations of the cell cycle.

AM-V9003M pFucci-G1 Orange is a mammalian expression vector encoding CoralHue ® monomeric Kusabira-Orange 2 (mKO2) fused to a part of human Cdt1
(hCdt1: Cdc10 dependent transcript 1).
Cdt1 is the DNA replication licensing factor. It accumulates during G1 phase in nuclei, but is degraded during S/G2/M phases by ubiquitin-mediated proteolysis.
A part of hCdt1 (30-120) is also degradable in a cell cycle dependent manner.

CoralHue ® mKO2 has been generated from dimeric CoralHue ® Kusabira-Orange (KO). CoralHue ® mKO2 has a feature of rapid maturation.