MPO, Human, ELISA kit

DATA SHEET

The human MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MPO. Biotinylated tracer antibody will bind to captured human MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MPO standards (log). The human MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.