pFucci-S/G2/M Green (Cloning vector)
Target | pFucci-S/G2/M |
---|---|
Product Type | Vector |
Formulation | Dry form. Reconstitute with distilled water or TE before use. |
Research Area | Drug Discovery, Fluorescent Proteins, Vector |
Description/Background | AM-V9014M pFucci-S/G2/M Green is a Cloning vector encoding CoralHue humanized-codon monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). “Fucci” stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-110) is also degradable in a cell cycle dependent manner. CoralHue hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue monomeric AG1 (mAG1) has been generated from tetrameric CoralHue Azami-Green (AG). |
Storage Temperature | -20°C |
Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |