EDN ELISA Kit

DATA SHEET

The EDN ELISA Kit measures Human EDN by sandwich ELISA. This ELISA detects Human EDN with a minimum detection limit of 0.62 ng/mL and does not cross-react with ECP.

Samples to be measured or standards are incubated in the wells coated with anti-human EDN monoclonal antibody. After washing, the peroxidase conjugated anti-human EDN polyclonal antibody is added into the microwells and incubated. After another washing, the peroxidase substrate is mixed with the chromogen and allowed to incubate for an additional period of time. An acid solution is added to each microwell to terminate the enzyme reaction and to stabilize the color development. The optical density (O.D.) of each microwell is measured at 450 nm using a microplate reader. The concentration of Human EDN is calibrated from a dose response curve based on the reference standards.