Anti-Phospho-DNA Topoisomerase IIα (Thr1342) (Human) mAb (Monoclonal Antibody)

Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo II alpha (170 kDa) and Topo II alpha (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II alpha is expressed constantly throughout cell cycle, whereas the expression of Topo II alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. It is considered that Topo II alpha plays an essential role in cell proliferation, especially during late S to M phase. Threonine 1342 in human Topo II alpha is phosphorylated throughout the cell cycle. Phosphorylation level of threonine 1342 in G2 to M phase is as twice as much in G1 or S phase. Anti-phospho-DNA Topoisomerase II alpha antibody recognize phosphorylated threonine residue, PT 1342 in human Topo II alpha specifically and does not react with non-phosphorylated form.