Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor™ 488
Monoclonal antibody of 50 µl targeting GFP for ICC.
| Target | GFP |
|---|---|
| Product Type | Antibody |
| Application | ICC |
| Clonality | Monoclonal |
| Conjugate | Alexa Fluor® 488 |
| Isotype | IgG2a ĸ |
| Immunogen | GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP |
| Host Species | Rat |
| Formulation | 50 µg of IgG in 50 µL volume of PBS containing 1% BSA and 0.09% NaN3. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain. |
| Research Area | Epitope Tag, Fluorescent Proteins, Purification/Separation, Smart-IP |
| Description/Background | Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Monoclonal anti-GFP antibody can detect GFP fusion protein on Immunocytochemistry. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at 4°C. |
| Protocols | ICC |
| Clone Number | RQ2 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone RQ2) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Wister rat lymph node immunized with GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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