Anti-Phospho-MBS (MYPT1) (Thr853) pAb
Polyclonal Antibody of 50 µg targeting MBS for ELISA, WB.
| Target | MBS |
|---|---|
| Product Type | Antibody |
| Application | ELISA, WB |
| Clonality | Polyclonal |
| Conjugate | Unlabeled |
| Isotype | IgG |
| Immunogen | a synthetic phosphopeptide corresponding to residues surrounding Thr853 of human MBS/MYPT1 (KLH-conjugated ) |
| Host Species | Rabbit |
| Species Reactivity | Human, Chicken, Mouse, Rat |
| Formulation | Supplied in 20 mM phosphate buffer (pH 7.5), 300 mM NaCl, 50 % glycerol. |
| Research Area | Cell Biology, Drug Discovery, Signal Transduction |
| Description/Background | The expression of a constitutively active form of Rho-kinase induced stress fiber and focal adhesion formation in fibroblasts and an increase in the level of myosin light chain (MLC20) phosphorylation. These results in smooth muscle and in non-muscle cells are attributed to an increase in MLC20 phosphorylation and this is thought to reflect the inhibition of myosin phosphatase (MP). MP is composed of three subunits: a catalytic subunit of type 1 phosphatase delta isoform, PP1c delta, and two non-catalytic subunits, 110 and 20 kDa. The 110 kDa subunit is a targeting molecule and thus has been termed myosin phosphatase target subunit 1 (MYPT1) or myosin-binding subunit (MBS). MBS/MYPT1 is the key molecule involved in regulation of MP activity. Phosphorylation by Rho-kinase inhibited MP activity and this reflected a decrease in Vmax. Activity of MP with different substrates also was inhibited by phosphorylation. Two major sites of phosphorylation on chicken MBS/MYPT1 were Thr696 and Thr853. Various point mutations were designed for these phosphorylation sites. Following thiophosphorylation by Rho-kinase and assays of phosphatase activity it was determined that Thr695 was responsible for inhibition. Rho-kinase, which is activated by GTP-RhoA, phosphorylated MBS/MYPT1 and consequently inactivated myosin phosphatase (MP). Over-expression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS/MYPT1 and MLC20. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. |
| Storage Temperature | Store at -20°C |
| Protocols | ELISA, WB |
| Sensitivity | Phospho-MBS/MYPT1 Thr853 Antibody detects endogenous MBS/MYPT1 only when phosphorylated at threonine853. The antibody does not recognize other myosin phosphatase regulatory subunit. |
| Molecular Weight | 135 kDa |
| Concentration | 1.0 mg/mL |
| Source | This polyclonal antibody is produced by immunizing rabbit with a synthetic phosphopeptide corresponding to residues surrounding Thr853 of human MBS/MYPT1. |
| Purification | IgG is purified by peptide-Sepharose affinity chromatography. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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