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Anti-Human CD16 (Clone 3G8) – Purified in vivo Functional Grade – 5.0 mg

5.0 mg



ApplicationB, CyTOF®, FC, IHC FF, in vivo, IP
ConjugatePurified in vivo Functional Grade
IsotypeIgG1 κ
ImmunogenHuman PMN cells
Host SpeciesMouse
Species ReactivityHuman, Baboon, Chimpanzee, Cynomolgus Monkey, Marmoset, Pigtailed Macaque, Rhesus Monkey, Squirrel Monkey
FormulationThis monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Research AreaCancer, Immunology, In vivo
Description/BackgroundCD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
Storage TemperatureFunctional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
ProtocolsB, CyTOF®, FC, IHC FF, in vivo, IP
Clone Number3G8
Concentration≥ 5.0 mg/ml
Purity≥95% monomer by analytical SEC, >95% by SDS Page
Endotoxin levels< 1.0 EU/mg as determined by the LAL method
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
5.0 mg