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Anti-Mouse CD8a (Ly 2) – Purified in vivo Functional Grade – 100 mg

100 mg



ApplicationB, CyTOF®, Depletion, FC, IHC FF, in vivo, IP, PhenoCycler®, WB
ConjugatePurified in vivo Functional Grade
IsotypeIgG2a κ
ImmunogenMouse thymus or spleen
Host SpeciesRat
Species ReactivityMouse
FormulationThis monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Research AreaCancer, Immunology, In vivo
Description/BackgroundCD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.
Storage TemperatureFunctional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
ProtocolsB, CyTOF®, Depletion, FC, IHC FF, in vivo, IP, PhenoCycler®, WB
Clone Number53-6.7
Concentration≥ 5.0 mg/ml
Purity≥95% monomer by analytical SEC, >95% by SDS Page
Endotoxin levels< 1.0 EU/mg as determined by the LAL method
Regulatory StatementFor Research Use Only. Not for use in diagnostic procedures.
100 mg