Anti-Phospho-STAT3 (Tyr708) mAb
Monoclonal antibody of 100 μL targeting STAT3 for WB.
This antibody reacts with human and mouse STAT3 phosphorylated at Tyr705, and zebrafish STAT3 phosphorylated at Tyr708.
| Target | STAT3 |
|---|---|
| Product Type | Antibody |
| Application | WB |
| Clonality | Monoclonal |
| Conjugate | Unlabeled |
| Isotype | IgG1 |
| Immunogen | 12 peptides containing the phosphorylated Tyr708 of zebrafish STAT3 (CVTQPpYLKTKFI) (703-714 aa) |
| Host Species | Mouse |
| Species Reactivity | Human, Mouse, Zebrafish |
| Formulation | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained. |
| Research Area | Cancer, Cell Biology, Signal Transduction |
| Description/Background | STAT (Signal Transducers and Activators of Transcription) proteins play important roles in development, cell differentiation and cell cycle control. STAT3 is an ~85 kDa protein involved in the signaling pathways of many cytokines and growth factors, including G-CSF and IL-6, where it functions as a negative regulator of transcription. STAT3 is also constitutively activated in a number of human tumors and it possesses anti-apoptotic activity and oncogenic potential. STAT3 may also regulate apoptosis by inhibiting NF-κB. Activation of STAT3 by tyrosine phosphorylation results in dimerization, nuclear translocation and DNA binding. |
| Storage Temperature | This antibody solution is stable for one year from the date of purchase when stored at -20°C. |
| Protocols | WB |
| Clone Number | PS3/1 |
| Concentration | 1 mg/mL |
| Source | This antibody was purified from hybridoma (clone PS3/1) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/c mouse splenocyte immunized with the 12 amino acids peptide CVTQPpYLKTKFI (703~714 aa) which contained the phosphorylated tyrosine 708 of zebrafish STAT3. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
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