T-Select Human CD1d Tetramer – APC
The α3 mutation significantly reduces the binding of MHC tetramer to human CD8. To minimizes the aberrant effect of some CD8 antibodies; clone SFCI21Thy2D3 (also known as “T8”) is recommended.
Target: NKT cells
Product Type: Tetramer
Application: FCM
Conjugate: APC
Class: CD1d
Species Reactivity: Human
Allele: CD1d
Research Area: Immunology, Immune Monitoring
Protocols: FCM
Specificity: NKT cells
Formulation: The reagent is dissolved in aqueous buffer that contains 10 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5 mM EDTA, 0.2% BSA and 0.09% NaN3. *Azide may react with copper or lead in plumbing system to form explosive metal azides. Therefore, always flush plenty of water when disposing materials containing azide into drain.
Description/Background: Natural Killer T (NKT) cells, a type of T cell that plays a significant role in immune response, produces a large quantity of INF- and IL-4 in response to glycolipids that presented by CD1d molecules. In recent years, NKT cells are reported to play a part in diabetes and tumor immunity. Therefore, a technology allowing quantitative measurement of CD1d-positive NKT cells would be a useful tool for immunology and clinical laboratory examinations. The development of MHC Tetramer technology has provided a breakthrough in the ability to follow T cell populations defined by their antigen specificity. Tetramers have been used widely to obtain a detailed analysis of the distribution and frequency of conventional CD4+ and CD8+ antigen-specific T cells during a variety of immune responses. T-Select Human CD1d Tetramer is a reagent created by tetramerizing biotinylated human CD1d/2m complexes with streptavidin labeled phycobiliprotein. -galactosylceramide (-GalCer), a glycosphingolipid originally isolated from marine sponges, appears to be presented by CD1d to activate both human and mouse NKT cells. -GalCer loaded T-Select Human CD1d Tetramer is a highly specific reagent for detection of NKT cells. Measurement can be performed using isolated lymphocytes/monocytes.
Storage Temperature: Store protected from light at 2-8ºC. Do not freeze.
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
