QuickSwitch™ Quant H-2 Kb-BV421 and Peptides Bundle 2

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 145

Target: Chain A, SARS-CoV-2 spike glycoprotein (1060-1068), Chain A, SARS-CoV-2 spike glycoprotein (263-270), Chain A, SARS-CoV-2 spike glycoprotein (449-456), Chain A, SARS-CoV-2 spike glycoprotein (691-699), ORF1ab polyprotein SARS-CoV-2 (5385-5393), ORF1ab polyprotein SARS-CoV-2 (5573-5580)

Product Type: Peptide, Tetramer

Application: FCM

Class: Class I

Species Reactivity: Mouse

Peptide Sequence: AAYYVGYL, ITGLYPTL, SIIAYTMSL, VTQLYLGGM, VVFLHVTYV, YNYLYRLF

Research Area: Immunology, Immune Monitoring

Protocols: FCM

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.