QuickSwitch™ Quant H-2 Kb-APC and Peptides Bundle 1

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 141

Target: Chain A, SARS-CoV-2 spike glycoprotein (233-241), Chain A, SARS-CoV-2 spike glycoprotein (325-332), Chain A, SARS-CoV-2 spike glycoprotein (447-455), Chain A, SARS-CoV-2 spike glycoprotein (511-518), Chain A, SARS-CoV-2 spike glycoprotein (539-546), Chain A, SARS-CoV-2 spike glycoprotein (902-909)

Product Type: Peptide, Tetramer

Application: FCM

Class: Class I

Species Reactivity: Mouse

Peptide Sequence: GNYNYLYRL, INITRFQTL, MAYRFNGI, SIVRFPNI, VNFNFNGL, VVLSFELL

Research Area: Immunology, Immune Monitoring

Protocols: FCM

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.