QuickSwitch™ Quant H-2 Kb Tetramer Kit-PE

Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 137.

Please use this link for access to the QuickSwitch™ Peptide Exchange Calculator to check your binding affinity.

Target: Custom

Product Type: Tetramer

Application: FCM

Conjugate: PE

Class: Class I

Species Reactivity: Mouse

Allele: H-2Kb

Peptide Sequence: Custom

Research Area: Immunology, Immune Monitoring

Protocols: FCM

Specificity: Custom

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Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.