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Tetramer/APC -Qa-1b Qdm (AMAPRTLLL)
Anti-mouse CD8 clone choice is critical when mouse MHC I tetramers, as some clones, including 53-6.7, lead to high background staining of all CD8+ T cells, rather than the antigen-specific ones. For best results, clone KT15, available from MBL International in FITC (K0227-4)is recommended. Additional information can be found in the datasheet that accompanies mouse class I tetramers, as well as the following reference containing a protocol for performing a cross titration series for optimization: Current Protocols in Immunology, MHC-Peptide tetramers to Visualize Antigen-Specific T Cells, Section 17.3.23-24, Supplement 111
Target: CD94/NKG2
Product Type: Tetramer
Application: FCM
Conjugate: APC
Class: Class I, Non-Classical
Species Reactivity: Mouse
Allele: Qa-1b
Peptide Sequence: AMAPRTLLL
Research Area: Immunology, Immune Monitoring
Protocols: FCM
Specificity: Qdm
Storage Temperature: 4°C
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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