Anti-PCBP2 (Human) pAb (Polyclonal Antibody)

Polyclonal Antibody of 200 µl targeting PCBP2 for IP, RIP, WB.

Target: PCBP2

Product Type: Antibody

Application: IP, RIP, WB

Clonality: Polyclonal

Conjugate: Unlabeled

Isotype: Affinity Purified Ig

Immunogen: Internal region synthetic peptide binding KLH, human PCBP2

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Formulation: 200 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Research Area: Epigenetics

Description/Background: Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins, characterized by their triple KH structure and by their poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also mediate protein-protein interactions within the RNP complexes. It binds specifically to polypyrimidine tracts within the 3' UTR of target mRNAs through KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are predominantly expressed in the nucleus and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent in the cytoplasm. The most characterized gene regulated by PCBP1 and PCBP2 is the a-globin gene, and the accumulated α-globin mRNA is known to account for approximately 95% of total cellular mRNA during terminal erythroid differentiation. Specific binding of PCBPs to 3′ UTR of mRNA is associated with the stabilization of a-globin mRNA. Microarray analysis of human mRNAs present in a specific subset of PCBP2-RNP complexes also showed a potential involvement of PCBP2 in transcription factor expression via association with their mRNA; further, the enrichment of PCBP2 mRNA in PCBP2-RNP complexes suggested that PCBP2 expression may be subject to posttranscriptional autoregulation.

Storage Temperature: -20°C

Protocols: IP, RIP, WB

Concentration: 1 mg/mL

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.