Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Monoclonal Antibody of 200 µl targeting 2,2,7-trimethylguanosine (m3G/TMG) for IP, IC
Target: 2,2,7-trimethylguanosine
Product Type: Antibody
Application: ICC, IP
Clonality: Monoclonal
Conjugate: Unlabeled
Isotype: IgG2a ĸ
Immunogen: Carrier protein-conjugated trimethylguanosine (m3G/TMG)
Host Species: Mouse
Formulation: PBS containing 50% Glycerol (pH 7.2). No preservative is contained
Research Area: Epigenetics
Description/Background:
Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m3G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m7G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m3G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m3G-cap structure.
Storage Temperature: -20°C
Protocols: ICC, IP
Clone Number: 235-1
Concentration: 1 mg/mL
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.