Anti-LC3 pAb for My select sampler set (Polyclonal Antibody)

This LC3 antibody is validated for multiple applications (WB, IHC, ICC and IP) and has over 85 citations, including in research papers with more than 1,000 citations (Saitoh T et al. Loss of the autophagy protein Atg16L1 enhances endotoxin-induced IL-1beta production. Nature 456, 264-8 (2008)). This is a polyclonal antibody of 100 ul that is raised in rabbit and is reactive with human, hamster, mouse, rat.

More information about LC3 and autophagy can be found in our Blog. In addition you may download our Autophagy Pathway Poster.

Target: LC3

Product Type: Antibody

Application: FCM, ICC, IHC, IP, WB

Clonality: Polyclonal

Conjugate: Unlabeled

Isotype: IgG

Immunogen: recombinant Human LC3 (MAP1LC3B:1-120 a.a.)

Host Species: Rabbit

Species Reactivity: Human, Hamster, Mouse, Rat

Formulation: PBS/50% glycerol, pH 7.2

Research Area: Autophagy

Description/Background:

Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.

Storage Temperature: -20°C

Protocols: FCM, ICC, IHC, IP, WB

Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.